Pyreneisapolyaromatichydrocarbonwithstrongshort-wavelengthfluorescence.Unlikeotherfluorescentdyes,polyaromatichydrocarbonsarefluorescentprobeswithstrongsensitivitytomicroenvironment.Thus,itsfluorescenceisdifferentinpolar,andnonpolarenvironments.Othereffectscanalsobeobserved.
Whentwopyrenesareincloseproximity,theyformexcimers.Excimerformationcanbeeasilyobserved,andquantitativelyestimatedusingfluorescentspectra.
PyreneazideisareagentforeasypyreneClickchemistrylabelingofanyalkyne-bearingmolecule.Itallowstoturnanymoleculeintopyrene-bearingprobe.
Thisazidecontainshydrophilictriethyleneglycollinkertomitigateintrinsicpyrenehydrophobicity,andfacilitateattachmenttobiomoleculesinaqueoussolutions.
OtherpyreneazidesforClickChemistrylabelingareavailable(2,and3).
ChemDrawformat
Generalproperties
| Appearance: | yellowishsolid |
| Molecularweight: | 402.45 |
| Molecularformula: | C23H22N4O3 |
| Solubility: | solubleindichloromethane,chloroform,moderatelysolubleinDMSO,DMF,acetonitrile |
| Qualitycontrol: | NMR1H(95%) |
| Storageconditions: | Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight. |
Spectralproperties
| Excitationmaximum,nm: | 343;326;313;276;265;242;234 |
| Emissionmaximum,nm: | 377;397 |
